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The other key interest of the lab is super-resolution microscopy! Alistair Curd has developed some great software recently published in Nanoletters to find patterns in 3D super-resolution datasets: We used this technique to analyse nuclear pore data, DNA origami and our favourite cells, cardiomyocytes! Software is available here
We recently published the structure of the shutdown state of full length smooth muscle myosin in nature The structure shows how phosphorylation of the regulatory light chains could activate the myosin, and this is likely to be conserved for the non-muscle myosin isoforms. Take a look! We're continuing to work on the structure of function...
Two highly regarded researchers in the ‘muscle’ field, died this year – Professor John Trinick and Professor Gerald Offer. We are planning a meeting to honour their life and work, and look forward to future developments. Coiled coils, myosin, titin and striated muscle: A reflection on the contributions of John Trinick and Gerald Offer. January 10th...
The Advanced Imaging Centre (AIC) has many visitors from many different places, who come to use their advanced imaging equipment. We're here for iPALM, and its working well so far!
Our recent paper in Cell Reports (Makowska et al.,(2015) Cell Reports. 13, 2118-2125: link to article) shows that some myosins (myo1b, Myo9b, Myo10 and Myo18a) are overexpressed in metastatic prostate cancer. Specifically knocking down expression of each of these myosins result in the re-organisation of the actin cytoskeleton, and the type of reorganisation seen depends on...
Myosin 10 is required to form filopodia, thin actin rich protrusions and it accumulates at the tip of these filopodia. It uses a combination of diffusion and active translocation to find its way to the right place in the cell. Tracking fluorescent myosin 10 molecules, and plotting their positions shows the tracks that this myosin...
As part of a group working on the primary cilium, we have used dSTORM (direct Stochastic Optical Reconstruction Microscopy) on a home-built 3D set up at Leeds to image proteins in the transition zone. This example shows the protein RPGRIP1L. For more information see our recent paper Lambacher et al., (2016) TMEM107 recruits ciliopathy proteins...
We have begun using Affimers - small non-antibody binding proteins, in 'super-resolution microscopy'. Their small size (~2nm - 10 fold smaller than antibodies) places an unique dye molecule close to the target of interest, resulting in a smaller linkage error (see green plot, in panel D), and enables them to penetrate dense cytoskeleton to label more efficiently...
Applying Machine Learning to 3D single molecule localisation analysis in super-resolution microscopy Supervisors: Prof Michelle Peckham (Leeds), Dr Joanna Leng (Leeds). Closing Date is 14 May 2018! More information on the Find a PhD website Project Description Super-resolution imaging is an advanced form of microscopy that allows a 10 fold improvement in the resolution that can be...